Micropropagation, Characterization, and Conservation of Phytophthora cinnamomi-Tolerant Holm Oak Mature Trees

Micropropagation of Elaeagnus angustifolia from mature trees.

Multiple shoots were obtained from nodal segments of mature trees of Elaeagnus angustifolia L. cultured on MS medium (Murashige and Skoog 1962) supplemented with 0, 0.88 or 2.22 micro M N(6)-benzyladenine. When nodal segments taken from the in vitro proliferated shoots were cultured under the same conditions, additional multiple shoots were obtained. Rooting of the in vitro propagated shoots wa...

micropropagation of eucalyptus globulus through mature trees

eucalyptus globulus is an important rapid-growing trees in eucalypts that applied for agroforestry and tree improvement programs. it has been used as a source of pulp in paper industry, essential oil and honey production. asexual propagation with conventional methods such as cutting and graft wasnot possible or obtained success was very low, because of hard proliferation of adventitious roots. ...

The Phosphite Responsive Transcriptome of Phytophthora Cinnamomi

Conservation of genetic diversity in artificially regenerated holm oak (Quercus ilex L.) populations

Reforestation with autochthonous species should take into account the preservation of the variability and the geographical structure of genetic diversity in forest species. In order to provide empirical data, genetic composition of an artificial population of holm oak (Quercus ilex L.) and its natural seed-origin population in Andalusia were analysed with six microsatellite loci. The genetic di...

Micropropagation of mature tree of Citrus limon

A protocol for in vitro cloning of mature plants of Citrus limon using nodal shoot segments has been developed. Three to four shoots were induced per axillary meristems on MS medium amended with 9 μM BAP. Repeated transfer of the initial explants for up to six passages on MS medium [having 50% of the original concentrations of NH4NO3 and KNO3, and supplement of 250 mg L (NH4)2SO4] with 0.22 μM ...